Application and significance of circRNA and high throughput sequencing in different metastatic pathways of gastric cancer

In this study, we will explore the expression profile of circular RNA (cRNA) in gastric cancer tissues and plasma order to evaluate its potential as a molecular marker for detection cancer. Additionally, research cRNA normal tissue. Reverse transcription drop digital polymerase chain reaction (RT ddPCR), circRNA profiling, prediction circRNA-microRNA interactions, design method RT ddPCR, well analysis clinicopathological factors survival outcomes are all included recommended approach. The results indicate that some circRNAs have distinct pattern patients with addition, quantitative ddPCR approach was created, identification certain connected prognosis accomplished. study provide significant new insights into ability function not only diagnostic markers but also independent prognostic dye-based is shown be an effective economical technique circRNA.